Journal: Scientific Reports

Article Title: Role Of Hif2α Oxygen Sensing Pathway In Bronchial Epithelial Club Cell Proliferation

doi: 10.1038/srep25357

Figure Lengend Snippet: ( A ) Relative FoxM1 mRNA levels (normalized to those of Hprt) in the lungs of control mice exposed to normoxia (n =  4) or hypoxia 10% O 2 (n =  6) for 3 days or HIF2α -deficient mice exposed to normoxia (n =  3) or hypoxia 10% O 2 (n =  4) for 3 days. ( B ) Relative RELM-α , RELM-β , EGF and HGF mRNA levels (normalized to that of Hprt) in the lungs of control mice exposed to normoxia (n =  3) or hypoxia 10% O 2 (n =  4) for 3 days or HIF2α -deficient mice exposed to normoxia (n =  3) or hypoxia 10% O 2 (n =  4) for 3 days. ( C ) Western blot analysis of RELM-α and β –actin protein levels in the lungs of control or HIF2α -deficient mice exposed to normoxia or hypoxia (10% O 2 ) for 3 days. A representative western blot is shown. ( D ) RELM-α immunohistochemistry in lung sections of control or HIF2α -deficient mice exposed to normoxia or hypoxia (10% O 2 ) for 3 days. For ( A , B ) panels values are expressed as mean ±  SEM and n is the number of the animals analysed. Error bars show 95% confidence interval based on duplicated samples. One-way ANOVA Tukey’spost test was used for statistical analysis. *p <  0.05; **p <  0.01; ns (not significant). For ( D ) panel, scale bar: 50 μ m.

Article Snippet: Western blotting was performed using 15% SDS-polyacrylamide gels and membranes were probed with antibodies raised against β -actin (Santa Cruz, sc1616, 1:1000) and RELMα (Abcam, ab39626, 1:1000).

Techniques: Control, Western Blot, Immunohistochemistry